The RapiZyme Proteinase K Digestion Module is a sample preparation kit for rapid, non-specific enzymatic degradation of proteins. It includes Proteinase K, guanidine HCl as a denaturant, and TCEP as a reductant, facilitating protein degradation in various assays like oligonucleotide extraction from biofluids and tissues, and degrading capsid proteins from viral vector samples.
- Contains proteinase K, guanidine HCl, and TCEP.
- Active between pH 4 and 12, with optimal activity at 50°C to 60°C.
- Compatible with denaturants and surfactants.
- Free of exonucleases, endonucleases, and ribonucleases.
- Facilitates detergent-free digestion of proteins.
- Aids in the disruption of oligonucleotide/protein binding.
- Aids in viral vector digestion for genome integrity analysis.
- Digestion can be achieved in as little as 40 min.